pPICZα B

價格:800元

聯系方式:I47-825O-882O

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pPICZalpha B載體基本信息

出品公司: Invitrogen
載體名稱: pPICZalpha B, pPICZα B, pPICZalphaB, pPICZαB
質粒類型: 畢赤酵母蛋白表達載體
表達水平: 高拷貝
啟動子: AOX1
克隆方法: 多克隆位點,限制性內切酶
載體大小: 3597 bp
5' 測序引物及序列: 5´ AOX1:5´-GACTGGTTCCAATTGACAAGC-3´
3' 測序引物及序列: 3´ AOX1:5´-GCAAATGGCATTCTGACATCC-3´
載體標簽: C-Myc, C-His
載體抗性: Zeocin 博來霉素
篩選標記: HIS4
備注:
插入基因是必需包含起始密碼子ATG。
產品目錄號: V195-20
穩定性: 穩定 Stable
組成型: 組成型 Constitutive
病毒/非病毒: 非病毒

pPICZalpha B載體質粒圖譜和多克隆位點信息

pPICZalpha B載體圖譜


pPICZalpha B多克隆位點

pPICZalpha B載體簡介

pPICZα  A,B和C是3.6 kb的畢赤酵母蛋白分泌表達載體。表達的重組蛋白是融合蛋白,含有一個N-端多肽,編碼釀酒酵母(Saccharomyces cerevisiae)α-因子分泌信號。載體能夠在畢赤酵母中利用甲醇誘導的高水 平的表達目的蛋白,并且可以用在任何畢赤酵母中,包括X33,GS115菌株,SMD1168H,KM71H。

pPICZα  A,B和C系列載體包含以下元素:

•5'端含有AOX1啟動子的嚴格調控,利用甲醇誘導表達任何感興趣的基因(Ellis等,1985; Koutz等人,1989;tschopp等人,1987A)。

α-因子分泌信號能夠分泌性表達目的蛋白。

•Zeocin抗性基因在大腸桿菌和畢赤酵母都能用于篩選(Baron等人,1992; Drocourt等人,1990)。

•C-端含Myc和His標簽,可以用于檢測和純化重組蛋白。

•pPICZ A, B, C三種讀碼框使得可以將基因克隆入載體而不發生任何移碼突變。

pPICZalpha B載體序列

LOCUS       pPICZα B          3597 bp ds-DNA    circular SYN 05-二月-2013
DEFINITION  Pichia pastoris vector for methanol-inducible expression of a 
            secreted protein.
ACCESSION   .
VERSION     .
KEYWORDS    pPICZ(alpha) B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     This file is created by Vector NTI
            http://www.lcvmqc.icu/
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     Linearize with SacI, PmeI, or BstXI for integration at the AOX1 
            locus.
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="synthetic DNA construct"
                     /lab_host="Pichia pastoris"
                     /mol_type="other DNA"
     promoter        2..940
                     /gene="Pichia pastoris AOX1"
                     /note="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             941..1207
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /note="alpha-factor secretion signal"
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /note="This feature has 3 segments:
                     ???1:??941?..??997?/?#993366?/?presequence
                     ???2:??998?..?1195?/?#993366?/?pro?region
                     ???3:?1196?..?1207?/?#993366
                     Cleavage sites after bases 997, 1195"
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
     misc_feature    1206..1280
                     /note="MCS"
                     /note="multiple cloning site"
                     /note="color: #99ccff"
     CDS             1279..1308
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /note="color: #cc99b2"
                     /translation="EQKLISEEDL"
     CDS             1324..1341
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     terminator      1421..1667
                     /gene="Pichia pastoris AOX1"
                     /note="AOX1 terminator"
                     /note="transcription terminator for AOX1"
                     /note="color: #ffffff"
     promoter        1707..2093
                     /gene="S. cerevisiae TEF1"
                     /note="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        2101..2148
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter?"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2167..2541
                     /codon_start=1
                     /gene="Sh?ble from?Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      2607..2854
                     /gene="S. cerevisiae CYC1"
                     /note="CYC1 terminator"
                     /note="transcription terminator for CYC1"
                     /note="color: #ffffff"
     rep_origin      complement(2929..3517)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga aaagagaggc
     1201 tgaagctgca ggaattcacg tggcccagcc ggccgtctcg gatcggtacc tcgagccgcg
     1261 gcggccgcca gctttctaga acaaaaactc atctcagaag aggatctgaa tagcgccgtc
     1321 gaccatcatc atcatcatca ttgagtttgt agccttagac atgactgttc ctcagttcaa
     1381 gttgggcact tacgagaaga ccggtcttgc tagattctaa tcaagaggat gtcagaatgc
     1441 catttgcctg agagatgcag gcttcatttt tgatactttt ttatttgtaa cctatatagt
     1501 ataggatttt ttttgtcatt ttgtttcttc tcgtacgagc ttgctcctga tcagcctatc
     1561 tcgcagctga tgaatatctt gtggtagggg tttgggaaaa tcattcgagt ttgatgtttt
     1621 tcttggtatt tcccactcct cttcagagta cagaagatta agtgagacct tcgtttgtgc
     1681 ggatccccca cacaccatag cttcaaaatg tttctactcc ttttttactc ttccagattt
     1741 tctcggactc cgcgcatcgc cgtaccactt caaaacaccc aagcacagca tactaaattt
     1801 tccctctttc ttcctctagg gtgtcgttaa ttacccgtac taaaggtttg gaaaagaaaa
     1861 aagagaccgc ctcgtttctt tttcttcgtc gaaaaaggca ataaaaattt ttatcacgtt
     1921 tctttttctt gaaatttttt tttttagttt ttttctcttt cagtgacctc cattgatatt
     1981 taagttaata aacggtcttc aatttctcaa gtttcagttt catttttctt gttctattac
     2041 aacttttttt acttcttgtt cattagaaag aaagcatagc aatctaatct aaggggcggt
     2101 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     2161 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     2221 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     2281 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     2341 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     2401 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     2461 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     2521 gtggccgagg agcaggactg acacgtccga cggcggccca cgggtcccag gcctcggaga
     2581 tccgtccccc ttttcctttg tcgatatcat gtaattagtt atgtcacgct tacattcacg
     2641 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     2701 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     2761 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     2821 gaaggttttg ggacgctcga aggctttaat ttgcaagctg gagaccaaca tgtgagcaaa
     2881 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2941 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3001 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3061 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3121 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3181 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3241 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3301 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3361 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3421 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3481 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3541 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagatc
//

pPICZalpha B載體質粒圖譜pdf版和相關資料下載

pPICZalpha B載體應用舉例

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